Download excel pclp1/20/2023 ![]() ![]() ![]() This was accompanied by a significant decrease in the adhesion of glomerular epithelial cells to type IV collagen. PAN exposure resulted in dose-dependent decreases in α3 and β1 expression, both at the protein level and at the mRNA level. ![]() The cells were then incubated for another 48 h in media containing 0, 0.5, or 5.0 μg/ml PAN. Glomerular epithelial cells were seeded into plastic flasks and allowed to attach and proliferate for 48 h. T-SV 40 immortalized human glomerular epithelial cells were used to study PAN's effects on α3β1 expression, as well as that of podocalyxin and the slit diaphragm component ZO-1. The major integrin expressed by glomerular epithelial cells is α3β1, which mediates attachment of these cells to extracellular matrix proteins including type IV collagen. ![]() Because PAN injection into rats results in retraction of glomerular epithelial cell foot processes and glomerular epithelial cell detachment, it was hypothesized that PAN might alter the contacts between these cells and the glomerular basement membrane. Puromycin aminonucleoside (PAN)-induced nephrosis is a well-described model of human idiopathic nephrotic syndrome, but the mechanism of PAN's effect is not completely understood. ![]()
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